Olympus Stereomicroscope SZX16

Meeting the Challenge of Life Science Imaging

Designed for advanced research, the SZX16 stereo microscope’s darkfield and polarization capabilities, 0.3 numerical aperture, and large 16.4:1 zoom ratio enable you to image whole organisms down to fine microscopic and individual cell structures.

szx16

Advanced Stereo with Wide Zoom Ratio

Outstanding Optical Performance

SZX16's optics, which include observation tubes, zoom body, and objectives are fully apochromatic to reduce chromatic blur over the entire magnification range. The SDF (Super Depth of Focus) objectives can deliver clear and high-contrast images of virtually any specimen. The maximum NA of 0.3 produces a resolution of 900 linepairs per millimeter allowing clear visualization of fine microscopic cell and cell component structures. Such amazing resolution and peerless magnification makes work more efficient, more precise and reveals much more information from samples.

Wide 16.4:1 Zoom Ratio

The SZX16 boasts a wide zoom ratio of 16.4:1, enabling 7x-115x magnification with a 1x objective, and up to 230x magnification with a 2x objective. This wide zoom range makes it suitable for a wide range of research applications, with low-magnification macro views for dissection and specimen manipulation, and sharp high-magnification views for close observation of microstructures.

Wide 16.4:1 Zoom Ratio

Choice of Suitable Contrast and Observation Method

SZX2-ILLTQ/SZX2-ILLTS

With a slim 41.5 mm design, that is approximately half the thickness of previous halogen lamp transmitted light illumination bases, new LED transmitted light illumination bases have a lower height to enable a low eyepoint and easy access to base-mounted samples during observation and operation. The LED illumination base SZX2-ILLTQ with quad position turret enables the user to choose cartridges and to switch from brightfield (standard/high/low), oblique (standard/high/low), darkfield, polarized illumination, and shutter with a simple turn. A one position LED illumination base is also an option (SZX2-ILLTS). This makes the SZX16 a flexible all-in-one microscope for various samples and observation tasks. Another advantage of LED illumination is a cooler base surface, which is suitable for long duration manipulation of live specimens. Power consumption is lower than a conventional 30W halogen light source. A life cycle of over 60,000 hours significantly reduces operation costs.

SZX2-ILLTS

cartridge

C. elegans
Oblique high-contrast cartridge（SZX2-COBH）

Medaka
Medaka
Darkfield cartridge（SZX2-CDF）

Practical Design for Advanced Research

Ample Working Space for Manipulation

Any type of manipulation under a microscope requires an ample space between the top of the sample and the bottom of the objective for the tools. The objectives available for the SZX16 have large working distance, which enable the use of both manual and automated manipulation and injection tools. Moreover, since the front end of the high-magnification 1.6x and 2x objectives is tapered, the available movement range for manipulator is significantly increased.

Ample Working Space for Manipulation

Advanced Fluorescence Observation

The SZX16 is suitable as a fluorescence microscope for observing everything from whole organisms to nuclear detail, thanks to its precisely engineered optics and high NA. The advanced glass materials with low autofluorescence and new surface coatings greatly increase the transmission of light and consequently improve image clarity. Furthermore, these features result in an excellent S/N ratio and high fluorescence signal intensity. Full control of the excitation light is facilitated by the 5-position filter wheel, which enables flexible illumination for low-magnification, high-resolution imaging.

Furthermore, the newly designed near-vertical reflected light illuminator’s excitation light paths are independent from the observation paths, allowing for substantially improved excitation light efficiency. These features provide far brighter fluorescence observation than conventional stereomicroscopes at all magnifications. Transmitted light observation for verification of specimen outline is possible even under reflected light fluorescence observation.

SZX16 Filter Wheel

Fluorescence Imaging Samples

Double Objectives for 3.5x to 230x Zoom

With the parfocal objectives and two-position revolving nosepiece, switching between objectives is very quick and easy, requiring little refocusing. Therefore, with the 0.5x and 2.0x objectives, the uninterrupted magnification range is 3.5x - 230x. This represents an effective zoom ratio of 65.7:1.

Double Objectives

Ergonomic Observation Tube and Extendable Eyepoint Adjuster

The SZX2 series features observation tubes with an optimized convergence angle designed to enable users to make observations from a natural position that minimizes eye fatigue. This solution effectively eliminates eyestrain during long periods of observation.
Our long tilting trinocular tube (SZX2-LTTR) improves the ergonomics of our stereo microscopes. This trinocular can be adjusted from 5 to 45 degrees to facilitate a more natural head and neck position while working. In addition, the eyepoint adjuster (SZX2-EEPA) enables users to raise or lower the eyepoint within a 120 mm range to accommodate users of different heights. The combination of these ergonomic components helps reduce stress and fatigue by keeping users in a more natural posture while they’re working.

Convergence Angle

Tilting Observation Tube

Extendable Eyepoint Adjuster

Digital Imaging and Documentation

The SZX16 can also be equipped with a trinocular tube and Olympus DP series digital camera for high-resolution imaging and research documentation. DP series cameras offer the high sensitivity for a wide range of applications, including high-sensitivity fluorescence imaging, and can be easily controlled using Olympus cellSens software.

Digital Imaging and Documentation

cellSens Extended Focus Imaging

Create a single in-focus image from successive image planes as the focus knob is turned using the Extended Focus Imaging (EFI) function. EFI composite images can also be created directly from previously captured Z-stacks.

cellSens Extended Focus Imaging

Observation Method	Fluorescence (Blue/Green Excitations)		✓
	Fluorescence （Ultraviolet Excitations）		✓
	Simple Polarized Light		✓
	Brightfield		✓
	Darkfield		✓
	Oblique		✓
Zoom	Zoom Ratio		16.4
	Magnification Indication		0.7, 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8, 10, 11.5
	AS		Built-in
	Features		Zoom variable magnification system with parallel optical axis Zoom drive system: Horizontal handle Click-stop for various zoom positions incorporated(Manual)
Optics	Galilean Optical System		✓
Illuminator	Fluorescence Illuminator	Hg Lamp	✓
		Xenon Lamp	✓
		Light Guide Illumination	✓
Focus	Focusing Mechanism	Coarse/Fine Focus	✓
	Load Capacity		0-10.0kg/ 2.7–15.0kg/ 8.0–25.0kg
	Coarse Handle Stroke		80 mm 120 mm
	Coarse Handle Stroke per Rotation		36.8 mm
	Fine Handle Stroke per Rotation		0.77 mm
Revolving Nosepiece	Manual	Standard Type	✓
Observation Tubes	Widefield (FN 22)	Trinocular	✓
		Tilting Trinocular	✓
		Ergonomic Long Tilting Trinocular	✓ (Intermediate magnification is 1.25X)
	Tube Inclination Angle		5–45° 30°
	Trinocular Tube Light Path Selection (Camera : Observation)		50%:50%, 0%:100% 0%:100%, 100%:0%
	Interpupillary Distance Adjustment		51–76 mm 52–76 mm 57–80 mm
Extendable Eyepoint Adjuster			Height adjustment range: 30–150mm, (with a scale attached)
Stands	Standard Stand		✓
	Optional Bases and Stands	Quad Position LED Transmitted Light Illumination Base	✓
		Single Position LED Transmitted Light Illumination Base	✓
		Large Stand	✓
		Universal Stand	✓
Dimensions			268 (W) × 386 (D) × 413 (H) mm (Standard Set Configuration)
Operating Environment	Indoor Use	Ambient Temperature	0–40 ºC (32–104 ºF)
		Maximum Relative Humidity	30–90 %
Eyepiece			WHSZ10X-H FN 22* WHSZ15X-H FN 16* WHSZ20X-H FN 12.5* WHSZ30X-H FN 7* *with diopter adjustment and reticle
Objectives			SDFPLFL0.3X, WD 141 mm, NA 0.045 SDFPLAPO0.5XPF, WD 70.5 mm, NA 0.075 SDFPLAPO0.8X, WD 81 mm, NA 0.12 SDFPLAPO1XPF, WD 60 mm, NA 0.15 SDFPLAPO1,6XPF, WD 30 mm, NA 0.24 SDFPLAPO2XPFC, WD 20mm, NA 0.3
Fluorescence Illuminators			Near vertical fluorescence illuminator 5 position fluorescence filter turret Accepts excitation balancers