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Olympus Fluorescence Microscopes BX53

The LED illuminator for the BX53 is equivalent to or better than a 100 W halogen lamp, delivering brightness that’s appropriate for teaching or contrast methods. The BX53 microscope can be customized for different observation methods, such as phase contrast and fluorescence, with modular components.

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Flexible and Customizable

The LED illuminator for the BX53 is equivalent to or better than a 100 W halogen lamp, delivering brightness that’s appropriate for teaching or contrast methods. The BX53 microscope can be customized for different observation methods, such as phase contrast and fluorescence, with modular components.

 

Acquire Precise Images with X Line Objectives

These objectives combine improved flatness, numerical aperture, and chromatic aberration to deliver clear, high-resolution images with better color accuracy across the entire spectrum. The elimination of violet color aberration creates clear whites and vivid pinks, improving contrast and sharpness.

X Line Objectives

 

Bright LED Lighting Designed for Pathology and Laboratory

With spectral characteristics similar to halogen light sources, the LED illuminator enables you to view the purple, cyan, and pink colors important in clinical research applications while enjoying the long use life of an LED.

Bright LED Lighting Designed for Pathology and Laboratory

 

Digital Imaging Helps Simplify Your Work

From advanced research to capturing images for conferences, our digital microscope cameras and cellSens imaging software help ensure fluorescence imaging with a high signal-to-noise ratio.

Digital Imaging Helps Simplify Your Work

 

Upgrade to Motorized Components

Automatically record and share magnification setting information with the optional coded nosepiece or change observation methods with a single touch using the hand switch. Motorized options include:

  • Fluorescence cube
  • Filter wheel
  • Nosepiece
  • Condenser

Motorized Components

 

Bright Images in Multi-Head Configurations

The multi-head light path has been redesigned not only to orient all images the same way but also to allow the intense LED to provide clear, bright images for up to 26 participants.

MDO System BX53LED+MDO

 

Save Microscopy Data via Coded Units

Add an optional coded nosepiece to your BX53 microscope to automatically record and share magnification setting information for post-imaging treatments. The metadata is automatically sent to cellSens software, helping minimize mistakes and scaling errors

Save Microscopy Data via Coded Units

 

Even Fluorescence Across the Field of View

Fluorescence illuminators with a fly-eye lens provide homogenous illumination across the entire wavelength spectrum and simplify burner alignment.

Surface of Fly-eye Lens, Enlarged Image

Surface of Fly-eye Lens, Enlarged Image

 

Flexible 8-Position Illuminator

Easily changeable mirror units to provide flexibility for a wide variety of fluorescence specimens. The design reduces the need to replace mirror units for multicolor or FISH applications, further accelerating your work.

8-Position Illuminator

 

Mirror Units Optimized for Fluorescence

The fluorescence mirror units have advanced coatings that provide high transmission and steep cut-off slopes. The interior surfaces eliminate over 99% of stray light for high sensitivity and sharp color separation.

Mirror UnitsMirror Units

 

High-Transmission Objectives with Reduced Autofluorescence

UIS2 objectives with a high numerical aperture (NA) have chromatic aberration corrections to provide high resolution, even from faint fluorescence signals. Advanced coating technologies reduce autofluorescence and improve the signal-to-noise ratio for flat, high transmission over a wide wavelength.

High-Transmission Objectives

 

Condenser Reduces Back Reflections

During fluorescence imaging, the motorized universal condenser reduces back reflections and autofluorescence by swinging its top lens out, automatically closing its diaphragm to a minimum, and locating the wheel between two positions.

 

Comfortable Stage

Mechanical stages are coated with an abrasion-resistant ceramic and have a rackless, wire-driven design.

Comfortable Stage

 

Keep Your Arms Resting on the Desk

The stage handle extender enables you to keep your arms resting on the desk while you work. A rubber cap can be mounted to the handle, so you can control the stage with small force.

Keep Your Arms Resting on the Desk

Observation Method Brightfield  
  Darkfield  
  Phase Contrast  
  Fluorescence (Blue/Green Excitations)  
  Fluorescence (Ultraviolet Excitations)  
  Differential Interference Contrast  
  Polarized Light  
  Simple Polarized Light  
Illuminator Transmitted Köhler Illuminator LED Lamp
  Fluorescence Illuminator 100 W Mercury Lamp
    Light Guide Illumination
Focus Focusing Mechanism Stage Focus
Intermediate Magnification Changer Manual Terret  
Revolving Nosepiece Motorized (7 positions)  
  Manual Coded (7 positions)
    Standard (7 positions)
Stage Manual Manual Stages with Right-Hand Control
  • X: 76 mm (3 in.), Y: 52 mm (2 in.)
  Mechanical Oil Rectangular Stage with Right-Hand Control
  • X: 76 mm (3 in.), Y: 52 mm (2 in.)
    Plain Stage Dimensions: 180 mm × 150 mm (7 in. × 6 in.)
    Rotatable Graduated Stage
  • Rotation: 360 degrees
Condenser Motorized Universal Condenser NA 0.9/ W.D. 1.5 mm for 1.25X–100X [swing-out: 1.25X–4X, with oil top lens: (NA 1.4/ W.D. 0.63 mm)]
  Manual Universal Condenser NA 0.9/ W.D. 1.5 mm for 1.25X–100X [swing-out: 1.25X–4X, with oil top lens: (NA 1.4/ W.D. 0.63 mm)]
    Swing-Out Condenser NA 0.9/ W.D. 2 mm (1.25X–100X)
    Achromatic/Aplanatic Condenser NA 1.4/ W.D. 0.7 mm (oil) (10X–100X)
    Darkfield Condenser Dry NA 0.8–0.92/ W.D. 4.52 mm (10X–40X)
    Darkfield Condenser Oil NA 1.2–1.4/ W.D. 0.5 mm (20X–100X)
    Low Magnification Condenser NA 0.75/ W.D. 1.55 mm (2X–100X)
    Abbe Conenser NA 1.1/ W.D. 0.7 mm (oil) (4X–100X)
    Phase Contrast Condenser NA 1.1/ W.D. 0.7 mm (4X–100X)
    Polarizing Condenser NA 0.9/ W.D. 1.3 mm (slide glass 1.5 mm) (4X–100X)
Observation Tubes Widefield (FN 22) Binocular
    Tilting Binocular
    Trinocular
    Tilting Trinocular
    Ergonomic Tilting Binocular
    Tilting, Telescopic, Lifting Binocular
    Trinocular for Infrared
    Erected Trinocular
    Erected Ergonomic Tilting Binocular
  Super Widefield (FN 26.5) Trinocular
    Erect image tilting trinocular
Dimensions (W × D × H)     274.5 mm × 614 mm × 469 mm (10.8 in. × 24.2 in. × 18.5 in.) (Epifluorescence configuration)
Weight     21 kg (46.3 lb) (Epifluorescence configuration)
Operating Environment Indoor Use Ambient Temperature 5 °C–40 °C (41 °F–104 °F)
    Maximum Relative Humidity 80% for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70% at 34 ºC (93 ºF), 60% at 37 ºC (99 ºF), to 50% relative humidity at 40 ºC (104 ºF)
    Supply Voltage Fluctuations ±10 %

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